Contact an Account ManagerDetermination of the bisphosphonate pamidronate disodium in urine by precolumn derivatization with fluorescamine HPLC and fluorescence detection
Summary
Type: HPLC
Application #: 105150
Catalog#: 720023.40 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4x250mm
Detection: fluorescence, excitation 395nm, emission 480nm
Conditions: eluent: 1mM aqueous Na2EDTA, adjusted to pH 6.5 with 1M NaOH - methanol (97:3, v/v)
flow rate 1ml/min
Authors: Flesch G
Source: J Chromatogr 489 (1989) 446 - 451
Details
Legend: Fig. 1. Chromatograms of A) drug-free human urine, B) drug-free dog urine and C) urine from a dog receiving a single intravenous injection of 5mg of pamidronate disodium in 0.15M saline.